Reagents and Preparation
1 gram toluidine blue in 100 ml of 50% isopropanol
Staining Procedure
1) Deparaffinize the slide by heating in hot incubation.
2) To remove paraffin, the slide has to be dipped in xylene for 1-2 minutes. Repeat the process four times.
3) Keep the slide in alcohol for 1-2 minutes to remove xylene. Repeat the process two times.
4) Wash the slide in running water.
5) Stain in a toluidine blue solution for 30 minutes at 37°C.
(Keep the slide in a petridish, fill the petridish with the stain, & keep it in incubator.)
6) Blot the section carefully.
7) Place in 50% isopropanol for 1 minute.
8) Clear in xylene & mount.
Result
Amyloid and many other tissue components stain an orthochromatic blue color, but when examined with a polarizing microscope, amyloid gives a dark red birefringence.
Image source: Arkana Laboratories